babesia

Infecção por theileria equi e babesia caballi em asininos da raça Zamorano-Leonês na província de Zamora (Espanha)

Infection by theileria equi and babesia caballi in Zamorano-Leonês donkeys in Zamora Province (Spain)

In the west of the Zamora Province in Castilla y León region, one of the most rural and isolated areas of Spain, the Zamorano – Leonés donkey – a native endangered breed -is still playing a central role in the traditional daily agriculture activities practiced by the local inhabitants. In February 2010 a study was carried out to understand the prevalence of equine piroplasmosis in the population of Zamorano – Leonés Donkey, collecting blood samples from 86 animals in 13 villages in the Zamora Proviince. Equine piroplasmosis is a tick-borne disease of equids, caused by Theileria equi and Babesia caballi. These intraerythrocytic parasites are responsible for a high morbidity and mortality in equids. The cELISA tests (competitive – inhibition Enzyme-Linked Immunosorbent Assay) revealed a positive seroprevalence of 21%, with 8 of the 86 donkeys positive for Theileria equi (9.3%), 10 for Babesia caballi (11.7%) and one of these 18 animals positive for both. The results of this study clearly demonstrate the importance of equine piroplasmosis affecting the Zamorano – Leonés donkey population. As far as we know this is the first study on intra-erythrocytic parasites in endangered Iberian breeds of donkeys and the results obtained reveal its importance in order to preserve this unique genetic heritage.

Journal

Acta Parasitológica Portuguesa

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Publication date

1 March 2010

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Semi-longitudinal study of trypanosomiasis and its vectors in donkeys (equus africanus asinus, fitzinger) in the Lamu archipelago

Phoebe Mukiria

Raymond Mdachi

J. Thuita

James Mutuku

Kennedy Wanjala

J. Omolo

Mulugeta Getachew

Andrew F. Trawford

Johnson Ouma

Grace Murilla

12th KARI Biennial Scientific Conference

Presentation date

8 November 2010

Kenya is home to some 600,000 donkeys that are found virtually in all ecological zones where they provide transport and draught power. In the Lamu archipelago where there is no motorised transport, donkeys are virtually the only available means of transport helping to transport farm produce, building materials and for getting from one place to the next. This study was conducted in April (dry season) and November 2009 (wet season) in four villages in Pate Island to investigate the prevalence and species of trypanosomes infecting donkeys and to identify the fly vectors playing a role in the transmission of trypanosomosis. Blood samples were collected from 288 and 319 donkeys and examined by buffy coat technique (BCT) and Giemsa stained blood smears. Trypanosomes were encountered in 3.1 and 7.5% of the examined donkeys in the dry and wet season respectively and there was no difference in the prevalence between villages during both seasons (p=0.159 and 0.709) but there was a significant difference (p=0.006) between seasons. Three species of trypanosomes detected were, in order of predominance were Trypanosoma congolense Broden (68.7%), Trypanosoma vivax Ziemann (21.8%) and Trypanosoma brucei Plimmer and Bradford (6.2%). Another 6.2% were mixed infections. There difference in mean PCVs between trypanosome infected and non-infected donkeys dry and wet seasons ranging significant to highly significant (p<0.05 and p<0.001) respectively. Trypanosome infection had a significant effect on mean body condition score of the donkeys during both seasons (p<0.05 and p<0.001). Prevalence of trypanosome infection was found to be independent of sex and age. However, mean PCV was significantly associated with age, sex and body condition scores. The entomological surveys revealed the presence of Glossina pallidipes Austen and other biting flies namely Stomoxys spp Linnaeus, Tabanus spp. Linnaeus, and Haematopota spp Linnaeus. Though the use of BCT for detection of trypanosomes in the field is almost universal, it has limited application especially in chronic infections and the field samples are in the process of being analysed using PCR to give a more accurate picture of the prevalence and as it relates to health and productivity of donkeys.

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Not published as conference proceedings