pain

Objective assessment of chronic pain in donkeys using the Donkey Chronic Pain Scale

Objective

To clinically evaluate previously developed pain scales [Donkey Chronic Pain Composite Pain Scale (DCP-CPS), Donkey Chronic Pain Facial Assessment of Pain (DCP-FAP), and combined Donkey Chronic Pain Scale (DCPS)], including behavioural and facial expression related variables, for the assessment of chronic pain in donkeys.

Study design

Prospective, blinded clinical study.

Animal population

A group of 77 donkeys (34 patients and 43 healthy control animals)

Methods

Animals were assessed by two observers that were blinded to the condition of the animals.

Results

Both DCP composite pain scale (CPS), DCP facial assessment of pain (FAP) and resulting Donkey Chronic Pain Scale (DCPS) scores showed good inter-observer reliability (Intra Class Correlation (ICC) coefficient = 0.91, confidence interval (CI) = 0.86–0.95, p < 0.001; ICC = 0.71, CI = 0.50–0.83, p < 0.001 and ICC = 0.84, CI = 0.72–0.91, p < 0.001 respectively). All scores (DCP-CPS, DCP-FAP and the resulting combined DCPS) were significantly higher for patients compared to controls at all time points (p < 0.001 for all three scales). Sensitivity and specificity for identification of pain (cut-off value > 3) was 73.0 and 65.1% for DCP-CPS, while for DCP-FAP this was 60.9% and 83.3%, respectively. For the combined DCPS, sensitivity was 87.0% and specificity 90.9% (cut-off value > 6).

Conclusions and clinical relevance

The DCPS, based on behavioural and facial expression-based variables, proved a promising and reproducible tool to assess different types of chronic pain in donkeys. The combination of behavioural and facial expression-based variables showed the best discriminatory characteristics in the current study. Further studies are needed for refinement of these tools.

Publication date
Research output

Objective assessment of chronic pain in donkeys using the donkey chronic pain scale (DCPS): A scale-construction study

Assessment of chronic pain is very important for monitoring and improving welfare and quality of life in donkeys. Freedom from disease and pain is one of the ‘five freedoms’ underlying animal welfare. The aim of the current study was to develop a pain scale for assessment of chronic pain in donkeys (Donkey Chronic Pain Scale; DCPS), including behavioural and facial expression-related parameters. The scale was applied to 77 donkeys (38 donkeys diagnosed with chronic health problems by means of clinical examination and additional diagnostic procedures and 39 healthy control animals). Animals were assessed twice daily for three consecutive days by two observers that were not masked to the condition of the animals but were unaware of the analgesic treatment regimens. Both composite, facial expression-based and combined DCPS pain scales showed excellent inter-observer reliability (Cronbach’s alpha = 0.98, 0.96 and 0.98 respectively; P < 0.001). Individual composite and facial expression-based pain scores and the resulting combined DCPS showed significant differences between donkeys with chronic conditions and control donkeys at all time points (P < 0.001). A DCPS cut-off of 6 showed good sensitivity and specificity (92% and 82.5% respectively) for presence of a chronic painful condition. Facial expression-related parameters separately showed low sensitivity. In conclusion, it is possible to use a composite pain scale for assessment of chronic pain in donkeys, based on behavioural and facial expression-based parameters. Further studies are needed to validate this pain scale before it can be used in veterinary practice.

Volume
267
Publication date
Research output

Monitoring acute pain in donkeys with the Equine Utrecht University Scale for Donkeys Composite Pain Assessment (EQUUS-DONKEY-COMPASS) and the Equine Utrecht University Scale for Donkey Facial Assessment of Pain (EQUUS-DONKEY-FAP)

Objective pain assessment in donkeys is of vital importance for improving welfare in a species that is considered stoic. This study presents the construction and testing of two pain scales, the Equine Utrecht University Scale for Donkey Composite Pain Assessment (EQUUS-DONKEY-COMPASS) and the Equine Utrecht University Scale for Donkey Facial Assessment of Pain (EQUUS-DONKEY-FAP), in donkeys with acute pain. A cohort follow-up study using 264 adult donkeys (n = 12 acute colic, n = 25 acute orthopaedic pain, n = 18 acute head-related pain, n = 24 postoperative pain, and n = 185 controls) was performed. Both pain scales showed differences between donkeys with different types of pain and their control animals (p < 0.001). The EQUUS-DONKEY-COMPASS and EQUUS-DONKEY-FAP showed high inter-observer reliability (Cronbach’s alpha = 0.97 and 0.94, respectively, both p < 0.001). Sensitivity of the EQUUS-DONKEY-COMPASS was good for colic and orthopaedic pain (83% and 88%, respectively), but poor for head-related and postoperative pain (17% and 21%, respectively). Sensitivity of the EQUUS-DONKEY-FAP was good for colic and head-related pain (75% and 78%, respectively), but moderate for orthopaedic and postoperative pain (40% and 50%, respectively). Specificity was good for all types of pain with both scales (91%–99%). Different types of acute pain in donkeys can be validly assessed by either a composite or a facial expression-based pain scale.

Journal
Volume
10
Issue
2
Start page
354
Publication date

A novel approach to pain recognition in donkeys

Gabriela Olmos
Faith A. Burden
Presentation date

Pain due lesions and clinical conditions is one of the main welfare concerns of the more than 42 millions donkeys that presently exist in the world. Yet, the knowledge to gauge pain in donkeys is lacking, misunderstood and/or not validated (Ashley, 2005).

Pain (yes/no/uncertain) and its severity (VAS; no pain=0 to worst pain=100mm) was assessed in 403 donkeys’ ante-mortem (ATM) and post-mortem (PTM). Also behaviours/signs (BS) and pain related lesions (PRL) were assessed ATM and PTM, respectively. Using principal component analysis the more than 53 BS and 238 PRL observed were narrowed to 58 biologically meaningful component or groups (14 BS and 44 PRL components, respectively). Components were used as risk factors in multiple regression analysis to identify which BS and/or PRL are commonly used in clinician’s (veterinary/pathologist) decision making process to determine whether a donkey ‘is’ (i.e. ATM) or ‘was’ (i.e. PTM) in pain and its severity (mild to severe). Furthermore, multiple correlations were made to understand which BS relate significantly with specific PRL and how.

A cross tabulation between pain ATM and PTM, where pain related lesions are used as a quasi-gold standard of pain assessment; identify that 2 in 10 donkeys are wrongly assumed as in NO-PAIN. Moreover, only 43% of the donkey observations are used by clinicians to make their opinion on donkey pain and its severity (i.e. 7 BS and 18 PRL components were significantly associated with pain as stated by clinicians). Yet, multiple correlations showed 20 plausible biologically meaningful relationships between BS and PRL; some currently not used by clinicians.

This methodology, previously successfully used in humans (Gregory, 2010) is novel to donkey veterinary medicine and warrants further research to consolidate findings. Nonetheless, the achieved correlation list of behaviours vs. pathologies is a significant work with valid applications in donkey pain identification and prognosis.

References

F. H. Ashley, A. E. Waterman-Pearson, H. R. Whay (2005) Behavioural assessment of pain in horses and donkeys: application to clinical practice and future studies, Equine Vet Journal, 37(6), 565-575.

N. G. Gregory (2010) Relationships between pathology and pain severities: a review. Animal Welfare 19, 437-448.

A novel approach of pain recognition and assessment in donkeys: initial results

Gabriela Olmos
Ayin Q. Alvarado-Arellano
Nicole du Toit
Faith A. Burden
Neville G. Gregory
Presentation date

This paper proposes an approach to use pain-relevant pathologies to enhance our understanding of the clinical and behavioural signs of pain in donkeys and outlines initial results of this ongoing investigation.

The methodology is summarized as follows. Trained veterinary clinicians examined live donkeys under two situations: A) before being euthanized due to a terminal illness or reduced quality of life (n= 347 sedentary donkeys in UK; DU) or B) when about to be slaughtered in an abattoir (n=164 working donkeys in Mexico; DM). The animals that represent populations in terms of age (years) for DU and DM respectively, were: <5 = 0.5%, 31.7%; 5-15 = 4%; 67.7%; 16-20 = 6%, 0.6%; >20 = 89.5% and 0%. For sex were: stallions 0.5%, 44%; geldings 52.2%, 8%; females 47.3%, 48% for DU and DM respectively. The body condition was: <2 =18.5%, 44.7%; 2.5 – 3 = 56.5%, 54%; >3.5 = 25%, 1% for DU and DM respectively and the girth was: 115 cm ±SD 9.6, 112cm ±SD 7.3 for DU and DM respectively.

The clinical examination (CE) included the oral mucosa, heart & respiratory rate, rectal temperature, plus an evaluation of 6 demeanours and 47 behaviours/signs that could relate to pain. At this point an overall pain visual analogue score was derived (VAS 0cm = no pain to 10cm = the worst pain). At post-mortem (PM), lesions/pathologies were noted, grouped by system-organ/tissue, ranked (mild, moderate, severe) and classified according to the following potentially painful pathologies: 1) trauma, 2) inflammation, 3) over-distension 4) perforation/ rupture, 5) stripping/ ulceration, 6) adhesions, 7) swelling, 8) exposure of sub-chondral bone. From these observations a second VAS was produced.

Raw correlations from the two populations showed that donkeys given a higher VAS at CE and PM presented with a greater severity of lesions in more systems as well as a higher heart rate at CE than those donkeys with a lower PM VAS. Moderate to severe pain identified CE was often recognized as severe pain at the PM stage. These initial observations show promise, and so further analysis will be done to test the relationships between pain indicators and pain pathologies.

Published as conference proceedings
Publication date
ISBN (13-digit)
978-9-08-686179-8

A case study to investigate how behaviour in donkeys changes through progression of disease

Gabriela Olmos
Gemma McDonald
Florence Elphick
Neville G. Gregory
Faith A. Burden
Presentation date

Donkeys have a limited repertoire of non-specific signs displayed when in pain or sick. This study looked closely at donkey behaviour during the progression of different diseases with the aim of improving pain and sickness recognition.

Video footage of a group of 79 donkeys at The Donkey Sanctuary was obtained for 6 months; where 45 diseased cases observed. Due data completes, four cases were selected [Cases A) with respiratory disease due to herpes virus (n=2) and Cases B) end-stage cases (hyperlipaemia, n=1; chronic laminitis, n=1)] plus four healthy controls (n=4). Cases A were observed for 8hrs on day -10 and -1 prior to disease onset (day 0 = first veterinary visit) and during treatment (day 1, 5 and 10). Cases B were observed for 8hrs on day -7, -3 and on the day of euthanasia (day 0). Total time (minutes) performing 47 different behaviours were compared between (painful/sick vs. healthy) and within donkeys using chi-square or fisher’s exacts tests.

Diseased donkeys in cases A and B spent on average 10% more time (range, 3 - 17%, p<0.01) with a lowered head carriage compared to controls. Conversely, they spent 15% less time (range 6 - 34%, P<0.04) with their ears in combinations (i.e. each ear in opposite direction), thus meaning ears were more static and unresponsive. Ear changes were subtle but were the earliest indicators of pain/sickness in the observed donkeys. Cases B compared to the controls spent 31% more time in recumbency (range 7 - 60%, p<0.01), and 40% less time eating (range 1 - 64%, p<0.01). The reduction in total eating time was not substituted by any other oral behaviour (e.g. drinking, grooming, licking, and investigative behaviours), where drinking and grooming were greatly affected in the donkey with hyperlipaemia. Finally, abdominal effort was only observed in cases A and tended to reduce with time on treatment (p=0.06).

Donkeys are working animals of great importance worldwide, and these results highlight useful behavioural changes that can be used as monitoring signs of pain/sickness in these animals. The potential use of these signs warrants further studies in greater and more diverse donkey populations.

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